Tuesday, January 6, 2009

Eleven golden rules of quantitative RT-PCR

Udvardi MK, Czechowski T, Scheible WR.

This is an important paper for plant biologists, considering that "the use of the term ‘semi-quantitative’ (and hence, semi-quantitative assays from which quantitative conclusions are to be made) are not acceptable in The Plant Cell; instead, assays must be shown to be sufficiently quantitative to support a conclusion of changes in levels" (thus, you should use qPCR)

In this sense, the following paper should also be taken into account: 
Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Czechowski T et al. Plant Physiol. 2005 Sep;139(1):5-17